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Bioseparations of Proteins

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Bioseparations of Proteins

Ajit Sadana, Satinder Ahuja

ISBN 0126140405
Pages 346

This book covers the fundamentals of protein inactivation during bioseparation and the effect on protein processing. Bioseparation of Proteins is unique because it provides a background of the bioseparation processes, and it is the first book available to emphasize the influence of the different bioseparation processes on protein inactivation.
Bioseparation of Proteins covers the extent, mechanisms of, and control of protein inactivation during these processes along with the subsequent and essential validation of these processes. The book focuses on the avoidance of protein (biologicalproduct) inactivation at each step in a bioprocess. It compares protein inactivation exhibited during the different bioseparation processes by different workers and provides a valuable framework for workers in different areas interested in bioseparations.
Topics include separation and detection methods; estimates of protein inactivation and an analysis of this problem for different separation processes; strategies for avoiding inactivation; the molecular basis of surface activity and protein adsorption,process monitoring, and product validation techniques; and the economics of various bioseparation processes and quality control procedures.

Introduction. Steps in Bioseparation Processes. High Resolution Fractionation Processes. Protein Inactivation during Bioseparation. Adsorption Influence on Bioseparation and Inactivation. Process Monitoring and Validation. Applications and Economics of Bioseparation. Protein Inactivation Avoidance and Refolding during Bioseparation. Subject Index.